產(chǎn)品應(yīng)用
Characteristics
- Quality controlled kit including all strains, S9, media, positive controls - no inhouse quality control, no sterility control necessary
- Certificate of Analysis is provided
- Excel Calculation Sheet to support data Interpretation free of charge
- Compliant with ICH M7 allowing testing for very small quantities of genotoxic impurities, e.g. nitrosamines
- Suitable for volatile test compounds
- Number of tests corresponds to 5 x 480 agar plates.
- Standardized procedure
- Shipped at room temperature from external storage in Germany or from Switzerland
- High sensitivity and excellent correlation with agar plate test (see referenced publications)
- 4 times less test compound as compared to the agar plate test, optimized for screening purposes
- Less hands-on-time and simultaneous processing of several replicates
- Automation available - high throughput
- 3Rs - 12 fold less consumption of rat liver liver S9, significant less test animals sacrificed
- Less error prone due to easy colorimetric read-out
- Waste - Significant less plastic waste and thus reduced contaminated waste in Environment
- Technology transfer available
- Enough material to process strains individually
The Ames test is a bacterial mutation reversion assay which evaluates the mutagenic potential of chemical, pharmaceutical or cosmetic substances, food additives, food packaging material, mixtures, drinking-, waste water or soils. The bacterial strains carry several mutations that inactivate the gene involved in the synthesis of histidine (s.typhimurium) or tryptophan (E.coli), i.e. they grow only in a medium supplemented with either his or trp. Mutagens induce mutations which may restore the ability of the bacteria to re-synthesize the amino acid (his or trp) and to grow in medium without his or trp. Growth of bacteria induces a pH shift which is easily detected by the yellow collor due to the pH-indicator included in the medium.
